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By the 1960s, increasingly sophisticated gel electrophoresis methods made it possible to separate biological molecules based on minute physical and chemical differences, helping to drive the rise of molecular biology.
By the 1960s, increasingly sophisticated gel electrophoresis methods made it possible to separate biological molecules based on minute physical and chemical differences, helping to drive the rise of molecular biology.
DNA-based markers (1960s) Although allozymes can detect variations in DNA, it is by an indirect method and not very accurate. DNA-based markers were developed in the 1960s. These markers are much more effective at distinguishing between DNA variants. Today these are the most commonly used markers.
1930s – first reports of the use of sucrose for gel electrophoresis; moving-boundary electrophoresis ; 1950 – introduction of "zone electrophoresis" (Tiselius); paper electrophoresis; 1955 – introduction of starch gels, mediocre separation
In the early 1960s, he developed new applications for gel electrophoresis. He applied the technique to identify different versions of the same protein, reflecting different alleles for the same genetic locus, in fruit flies.
In medicine, protein electrophoresis is a method of analysing the proteins mainly in blood serum. Before the widespread use of gel electrophoresis , protein electrophoresis was performed as free-flow electrophoresis (on paper) or as immunoelectrophoresis.
Moving-boundary electrophoresis was developed by Arne Tiselius in 1930. [3] Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of colloids through electrophoresis, the motion of charged particles through a stationary liquid under the influence of an electric field.
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.
Gel electrophoresis of nucleic acids is an analytical technique to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules are placed on a gel, where an electric field induces the nucleic acids (which are negatively charged due to their sugar- phosphate backbone) to migrate toward the positively charged anode.
Discontinuous electrophoresis (colloquially disc electrophoresis [a]) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis. [2] [1] This method produces high resolution and good band definition. It is widely used technique for separating proteins according to size and charge. [3]