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  2. Paper chromatography - Wikipedia

    en.wikipedia.org/wiki/Paper_chromatography

    Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC).

  3. Thin-layer chromatography - Wikipedia

    en.wikipedia.org/wiki/Thin-layer_chromatography

    Related. HPTLC. Paper chromatography. Agarose gel electrophoresis. SDS-PAGE. Thin-layer chromatography ( TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2]

  4. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    They established the principles and basic techniques of partition chromatography, and their work encouraged the rapid development of several chromatographic methods: paper chromatography, gas chromatography, and what would become known as high-performance liquid chromatography. Since then, the technology has advanced rapidly.

  5. History of chromatography - Wikipedia

    en.wikipedia.org/wiki/History_of_chromatography

    Chromatography, literally "color writing", [1] was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll (which is green) and carotenoids (which are orange and yellow). New forms of chromatography developed in the 1930s and 1940s made the technique useful for a wide range ...

  6. Two-dimensional chromatography - Wikipedia

    en.wikipedia.org/wiki/Two-dimensional_chromatography

    Modern two-dimensional chromatographic techniques are based on the results of the early developments of paper chromatography and thin-layer chromatography (TLC) which involved liquid mobile phases and solid stationary phases.

  7. High-performance thin-layer chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_thin...

    High-performance thin-layer chromatography ( HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds. [1] This TLC-based analytical technique enhances compound resolution for quantitative analysis. Some of these improvements involve ...

  8. Retardation factor - Wikipedia

    en.wikipedia.org/wiki/Retardation_factor

    In chromatography, the retardation factor ( R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor RF is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [2]

  9. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Reversed-phase liquid chromatography ( RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [1] [2] [3] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the reversed ...

  10. Chromatography in blood processing - Wikipedia

    en.wikipedia.org/wiki/Chromatography_in_blood...

    Chromatography in blood processing. Chromatography is a physical method of separation that distributes the components you want to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. Cold ethanol precipitation, developed by Cohn in 1946, manipulates pH, ionic strength ...

  11. Affinity chromatography - Wikipedia

    en.wikipedia.org/wiki/Affinity_chromatography

    Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. The specific type of binding interaction depends on the biomolecule of interest; antigen and antibody, enzyme and substrate, receptor and ligand, or protein ...